“SINGLE-POINT” SEPHADEX GEL FILTRATION ASSAY FOR ESTIMATION OF RESERVE BILIRUBIN BINDING CAPACITY
نویسندگان
چکیده
منابع مشابه
Bilirubin binding affinity of serum: comparison of qualitative and quantitative sephadex gel filtration methods.
A simple, 30-mm method of estimating the binding of bilirubin to serum protein was compared with a quantitative method. Good correlation was found between the two methods, both based on Sephadex gel filtration. The results indicate that the presence of loosely bound bilirubin can be determined with this simple, qualitative, semimicro-scale test. This determination is important for the clinical ...
متن کاملFaster estimation of reserve bilirubin binding capacity of serum from the neonate by thin-layer chromatography on sephadex.
Knowledge of the reserve bilirubin-binding capacity is useful in management of hyperbilirubinemia in the newborn. We describe thin-layer gel chromatography that permits measurement of this variable, with a 250-mul sample of serum, within 50 min. Single samples from four different infants can be analyzed on the same plate. The capacity of 40 sera from jaundiced and of 19 sera from nonjaundiced i...
متن کاملThe routine use of Sephadex to estimate "free" bilirubin and the residual serum bilirubin binding capacity.
Those of your readers who are interested in anticonvulsant-drug analyses will be familiar with the drugs ethotoin (Peganone#{174}, 3-ethyl-5-phenylhydantoin) and mephenytoin (Mesantoin#{174}, 5-ethyl-3methyl-5-phenylhydantoin). They will also be familiar with the name Nirvanol#{174}. Unfortunately, a dreadful state of confusion exists among various authorities as to whether Nirvanol is ethotoin...
متن کاملPurification of human plasminogen and plasmin by gel filtration on Sephadex and chromatography on diethylaminoethyl-Sephadex.
Highly purified human plasminogen has been prepared from a variety of plasma fractions and by different purification methods. The two major fractions used have been serum or plasma euglobulin and Cohn plasma Fraction III. The purification methods (1-14) have all yielded products of high purity. Plasminogen useful for further purification and isolation studies can be prepared by the Kline method...
متن کاملEstimation of bilirubin binding capacity of neonatal serum.
One aim of laboratory investigation in neonatal jaundice is to prevent kernicterus from occurring. Estimation of the bilirubin-binding capacity of serum is an important measurement along with total bilirubin determination. With a convenient Sephadex competitive binding procedure, KERNLUTE provides information on whether or not the neonate's bilirubin-binding capacity has been exceeded. Further ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Pediatric Research
سال: 1974
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-197404000-00357